| Gene name: | ING1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//SAHF//Immunofluorescence |
| Description: | The results showed that 2BS cells overexpressing of p33ING1befficiently induced the expression of SA-β-gal activity, flattened senescent cell morphology, and SAHF DNA staining pattern which is indicated by co-localization with H3K9Me3. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | qPCR//Western blot//Luciferase reporter assay |
| Target gene description: | qPCR and Western blot results showed that introduction of p33ING1b upregulated p16INK4amRNA and protein levels.Moreover, p16INK4apromoter activity was upregulated by p33ING1b.The results showed that cells treated with p33ING1balone revealed strong SA-β-gal staining and p16 increasing, whereas cells doubly treated with p33ING1b and p16-shRNA reversed the senescence phenotypes. As expected, p16-shRNA-infected cells displayed young phenotypes. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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