| Gene name: | HMGA2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Gene ID: | 8091 |
| Category: | protein coding |
| Phenotype: | Retinoblastoma |
| Experimental category: | L |
| PMID: | 23969248 |
| Experiment: | SA-β-gal activity assay//qPCR//Western blot |
| Description: | We found that the ectopic overexpression of HMGA2 led to elevated SA-β-galactosidase staining and acquisition of the SAHF-like chromatin foci in the nuclei. Meanwhile, the expression levels of the senescence-related proteins, such as p16, p21, p53 and phosphorylated p53, were prominently increased. The transcription level of HDM2 that reduces the stability of p53 protein was decreased together with a slightly up-regulated p14ARF, an inhibitor of HDM2, in WI38 cells expressing HMGA2 . Apparently, these results indicate that HMGA2 can induce the senescence phenotypes in WI38 cells. |
| Target gene: | E2F |
| Official symbol(s): | E2F1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Surprisingly, both HMGA2 and Rb were detected in the same protein complex in our model (data not shown), together with the down-regulation of E2F target genes in WI38 cells in response to HMGA2 overexpression. |
| Regulatory pathway: | RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Western blot//qPCR//RT-PCR//Immunofluorescence |
| Pathway description: | To further test whether Rb is a crucial factor in SAHF formation induced by HMGA2, E7 protein was used as the specific inhibitor of Rb , and the effect of E7 protein on the degradation of Rb was confirmed by Western blotting . Interestingly, we found that E7 protein was able to partly abolish the SAHF formation induced by HMGA2, as revealed by cellular immunofluorescence and decreased percentage of cells with SAHF .Furthermore, as another inhibitor targeted to Rb, SV40 had a similar effect to decrease the HMGA2-induced SAHF formation . |
Annotation:
Loading,please wait...