| Gene name: | PRKAA1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NIH-3T3 |
| Experiment: | SA-β-gal activity assay |
| Description: | Next, we observed that when the H2O2-treated cells were incubated with medium containing Met and BBR, there was a dose-dependent decrease in the percentage of SA-β-Gal-positive cells, which was similar to that caused by rapamycin treatment.As expected, when combined with CC, the effects of Met or BBR on senescence prevention were largely blunted when CC was coexisted, as indicated by the remarkable increase in SA-β-Gal-positive cells. |
| Target gene: | LC3//P62 |
| Official symbol(s): | MAP1LC3A//P62 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | With Western blot assay, we found that both Met and BBR alleviated the H2O2-induced accumulation of the LC3 and p62 proteins, and this alleviation was consistent with the effect of rapamycin. |
| Regulatory pathway: | MTOR |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | Moreover, we found that BBR treatment significantly suppressed mTOR phosphorylation in senescent cells, similar and even stronger than that induced by rapamycin and insulin as an mTOR activation control. |
Annotation:
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