| Gene name: | NLRX1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCCLM3 |
| Gene ID: | 79671 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 29482578 |
| Experiment: | CCK-8 assay//SA-β-gal activity assay |
| Description: | We found that NLRX1 overexpression could hinder cell proliferation in HCCLM3 cells, while knocking down NLRX1 in Huh7 resulted in significant higher proliferation rates. NLRX1-OE significantly increased SA-β-gal activity, indicating a higher proportion of aging in NLRX1-OE cells compared to cells transfected with vector. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | We found that NLRX1-OE could effectively upregulate P21 expression, and expressions of downstream targets of P21 including CDK1 and CDK2 were significantly repressed due to high level of P21 expression caused by NLRX1 overexpression, while knocking down NLRX1 in Huh7 resulted in increased expression of CDK1 and CDK2. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | NLRX1-OE markedly inhibited the phosphorylation of mTOR, indicating that NLRX1 suppresses PI3K-AKT signaling. |
Annotation:
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