About Enhancer
| Enhancer ID: | E_02_0943 |
| Species: | human |
| Position : | chr8:124323890-124325890   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Prostate cancer |
| Pubmed ID: | 22771493 |
| Enhancer experiment: | qPCR,Luciferase Reporter Assay,ChIP,EMSA,3C |
| Enhancer experiment description: | Taken together, these data demonstrate that the poten_x0002_tial ARBS identified within the distal enhancer region of ATAD2 gene indeed interacts strongly and specifically with AR in vitro and suggest that it could be a good candidate as a functional ARBS in vivo.To ascertain whether the ARBS identified within the ATAD2 enhancer region is functional in cells in vivo, we performed ChIP using formaldehyde cross-linked LNCaP cells. |
About Target gene
| Target gene : | ATAD2(ANCCA,CT137,PRO2000) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,EMSA,RT-qPCR |
| Target gene experiment description: | In addition, we analyzed chromatin immunoprecipita_x0002_tion (ChIP)-seq data from the literature to identify genes that might share the same regulation mechanism as ATAD2.With this in mind, we aimed first at checking whether ATAD2 might be a direct target gene of AR. To test this hypothesis, we analyzed ATAD2 gene response to androgens in LNCaP cells in the presence of cycloheximide (CHX), a protein synthesis inhibitor. |
About TF
| TF name : | AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR)E2F1(E2F-1,RBAP1,RBBP3,RBP3) |
| TF experiment: | EMSA |
| TF experiment description: | To check the binding of AR to this putative ARBS, we first performed EMSA experiments with bacterially produced AR-DNA-binding domain (DBD) purified to homogeneity,siE2F1 and siAR transfection showed the very efficient and specific knockdown of E2F1 and AR, respectively, at both mRNA and protein levels. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene