About Enhancer
| Enhancer ID: |
E_02_0913 |
| Species: |
human |
| Position :
|
chr7:116374406-116376406
  |
| Biosample name: |
|
| Experiment class :
|
Low throughput |
| Enhancer type: |
Enhancer |
| Disease: |
Melanoma |
| Pubmed ID: |
24443471
|
| Enhancer experiment: |
3C,ChIP |
| Enhancer experiment description: |
Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb enhancer as an anchor region. We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb enhancer and the MET TSS, demonstrating that this lineage-specific enhancer undergoes inducible chromatin looping in response to oncogene withdrawal in melanoma cells. |
About Target gene
| Target gene : |
MET(AUTS9,DFNB97,HGFR,RCCP2,c-Met) |
| Strong evidence: |
3C |
| Less strong evidence: |
ChIP |
| Target gene experiment description: |
Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb Enhancer as an anchor region.We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb Enhancer and the MET TSS. |
About TF
| TF name : |
MITF(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32) |
| TF experiment: |
ChIP-seq,3C |
| TF experiment description: |
To test the necessity of MITF for inducible chromatin looping between the MET +63-kb Enhancer and MET TSS in response to BRAF inhibition, 3C was performed in COLO829 cells in the presence or absence of PLX4032 and MITF depletion. |
About Function
| Enhancer function : |
This enhancer displays inducible chromatin looping with the MET promoter to up-regulate MET expression upon BRAF inhibition. |
| Enhancer function experiment: |
ChIP,3C |
Enhancer function
experiment description: |
ChIP and 3C analysis were performed on wild-type or Enhancer-edited cells treated with PLX4032.A significant decrease in both H3K27 acetylation,a histone modification associated with active Enhancer state,and Enhancer-promoter chromatin looping was observed in Enhancer-edited cells. |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs