About Enhancer
| Enhancer ID: | E_02_0901 |
| Species: | mouse |
| Position : | chr2:25423079-25423874   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24915132 |
| Enhancer experiment: | ChIP |
| Enhancer experiment description: | Incontrast, lung fibroblasts, which are of mesenchymal origin andwhich lack Fut7 mRNA and selectin ligand expression, carried adifferential histone modification pattern with an enrichment ofH3K27me3 at the CNS and the Fut7 gene locus and absenceof H3K4me2 marks. This suggests that the pattern of histone mod-ifications might control tissue-specific expression of Fut7, ratherthan the differentiation and activation dependent expression inT cells |
About Target gene
| Target gene : | Fut7(AI853193,FTVII,Fuc-TVII,FucT-VII) |
| Strong evidence: | -- |
| Less strong evidence: | qRT-PCR,Luciferase Reporter Assay |
| Target gene experiment description: | The CNS was cloned in different orientations in the Enhancer position of the pGL3 vector carrying the +728/+1523 construct in promoter position and transfected into Th1 cells. |
About TF
| TF name : | Ep300(KAT3B,MKHK2,RSTS2,p300) |
| TF experiment: | ChIP |
| TF experiment description: | As our histone ChIP data as well as analysis of genome-wide p300 binding data published by Vahedi et al.(2012) revealed distribution ofthe active histone mark as well as enrichment of p300 binding (data not shown) beyond the homology region, i.e. the CNS, we extended the CNS by about 300 bp upstream and 150 bp downstream of the original construct. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene