About Enhancer

Enhancer ID: E_02_0527
Species: human
Position : chr11:5279732-5280272
Biosample name:
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Erythrocytic leukemia
Pubmed ID:  30012865
Enhancer experiment: ChIP-seq
Enhancer experiment description: We generated a synthetic zinc finger DNA-binding domain (ZF) targeting the HBG-4kb HS. The HBG-4kb ZF interacted with the target site in vitro and in the context of cells with high affinity and specificity.

About Target gene

Target gene : HBG2(HBG-T1,TNCY),HBG2(HBG-T1,TNCY),HBG2(HBG-T1,TNCY)
Strong evidence: --
Less strong evidence: PCR
Target gene experiment description: The regions that we analyzed were the HBG-4kb HS,a 327 region located 4 kb upstream (HBG-8kb) or 1 and 2 kb downstream(HBG-3kb and HBG-2kb) of 328 the HBG-4kb HS,the G?-(HBG2) and A?-(HBG1) globin gene promoter regions.;The regions that we analyzed were the HBG-4kb HS,a 327 region located 4 kb upstream (HBG-8kb) or 1 and 2 kb downstream(HBG-3kb and HBG-2kb) of 328 the HBG-4kb HS,the G?-(HBG2) and A?-(HBG1) globin gene promoter regions.;The regions that we analyzed were the HBG-4kb HS,a 327 region located 4 kb upstream (HBG-8kb) or 1 and 2 kb downstream(HBG-3kb and HBG-2kb) of 328 the HBG-4kb HS,the G?-(HBG2) and A?-(HBG1) globin gene promoter regions.

About TF

TF name : USF1(FCHL,FCHL1,HYPLIP1,MLTF,MLTFI,UEF,bHLHb11)USF2(FIP,bHLHb12)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)MAFK(NFE2U,P18)NFE2(NF-E2,p45)USF1(FCHL,FCHL1,HYPLIP1,MLTF,MLTFI,UEF,bHLHb11)USF2(FIP,bHLHb12)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)MAFK(NFE2U,P18)NFE2(NF-E2,p45)USF1(FCHL,FCHL1,HYPLIP1,MLTF,MLTFI,UEF,bHLHb11)USF2(FIP,bHLHb12)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)MAFK(NFE2U,P18)NFE2(NF-E2,p45)
TF experiment: ChIP
TF experiment description: Reduced binding of transcription factors with the HBG-4kb HS in K562 cells expressing the HBG-4kb ZF. K562 cells (Untreated) or K562 cells exposed to 500 nM HBG-4kb ZF for 12 h were subjected to ChIP using antibodies specific for EGR1, USF1, USF2, NF-E2 (p45), MafK, or the negative control, IgG.;Reduced binding of transcription factors with the HBG-4kb HS in K562 cells expressing the HBG-4kb ZF. K562 cells (Untreated) or K562 cells exposed to 500 nM HBG-4kb ZF for 12 h were subjected to ChIP using antibodies specific for EGR1, USF1, USF2, NF-E2 (p45), MafK, or the negative control, IgG.;Reduced binding of transcription factors with the HBG-4kb HS in K562 cells expressing the HBG-4kb ZF. K562 cells (Untreated) or K562 cells exposed to 500 nM HBG-4kb ZF for 12 h were subjected to ChIP using antibodies specific for EGR1, USF1, USF2, NF-E2 (p45), MafK, or the negative control, IgG.

About Function

Enhancer function : the HBG-4kb HS site is important for fetal globin production and suggest that it may act by opening chromatin in a directional manner.
Enhancer function experiment: CRISPR/Cas9
Enhancer function
experiment description:		 Expression of the adult ?-globin, the embryonic -globin, and the GATA1 genes was not affected by the HBG-4kb ZF. Delivery of the Control ZF did not cause any change in expression of the ?-globin, ?-globin, -globin, or GATA1 genes.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
USF1 Signaling mediated by p38-alpha and p38-beta pid 35
USF2 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
MAFK Factors involved in megakaryocyte development and platelet production reactome 111
NFE2 Factors involved in megakaryocyte development and platelet production reactome 111
USF1 Signaling mediated by p38-alpha and p38-beta pid 35
USF2 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
MAFK Factors involved in megakaryocyte development and platelet production reactome 111
NFE2 Factors involved in megakaryocyte development and platelet production reactome 111
USF1 Signaling mediated by p38-alpha and p38-beta pid 35
USF2 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
MAFK Factors involved in megakaryocyte development and platelet production reactome 111
NFE2 Factors involved in megakaryocyte development and platelet production reactome 111

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs