About Enhancer
| Enhancer ID: |
E_02_0404 |
| Species: |
human |
| Position :
|
chr16:10440279-10442279
  |
| Biosample name: |
|
| Experiment class :
|
Low throughput |
| Enhancer type: |
Enhancer |
| Disease: |
-- |
| Pubmed ID: |
30327417
|
| Enhancer experiment: |
qChIP |
| Enhancer experiment description: |
We then analyzed peak A and Ciita promoter I by qChIP in NFAT5-deficient or control macrophages for different histone modifications that are enriched in gene enhancers (H3K4me1) or promoters (H3K4me3) and a modification that marks transcribed regions and active enhancers.We observed that peak A region had a slightly higher content in H3K4me1 compared with Ciita promoter I, and this was NFAT5 independent. |
About Target gene
| Target gene : |
Ciita(C2ta,EG669998,Gm9475,Mhc2ta) |
| Strong evidence: |
-- |
| Less strong evidence: |
ChIP-seq |
| Target gene experiment description: |
ChIP-seq analysis of wild-type and NFAT5-deficient BMDMs showing the position of NFAT5-binding site peak A 47 kb upstream of the Ciita locus. |
About TF
| TF name : |
Nfat5(AI225870,B130038B15Rik,CAG-8,CAG80,NFATL1,OREBP,TonEBP,mKIAA0827,nfatz) |
| TF experiment: |
qChIP |
| TF experiment description: |
We therefore asked whether NFAT5 bound these regulatory regions. Quantitative chromatin immunoprecipitation (qChIP) with a combination of two polyclonal antibodies to NFAT5 detected its specific binding to promoter I of Ciita and the promoter of H2-Ab both in untreated macrophages as well as in macrophages treated with IFN?.This result indicated that NFAT5 is a relevant factor to control Ciita expression in macrophages through its myeloid promoter I. Altogether,our analysis of primary myeloid and lymphoid APCs uncovered a selective requirement of NFAT5 in macrophages for expressing CIITA and MHCII. |
About Function
| Enhancer function : |
-- |
| Enhancer function experiment: |
-- |
Enhancer function
experiment description: |
-- |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs