About Enhancer
| Enhancer ID: |
E_02_0384 |
| Species: |
mouse |
| Position :
|
chr1:78074974-78076974
  |
| Biosample name: |
|
| Experiment class :
|
High+Lowthroughput |
| Enhancer type: |
Enhancer |
| Disease: |
-- |
| Pubmed ID: |
30453072
|
| Enhancer experiment: |
Immunohistochemical analysis?Real-time PCR |
| Enhancer experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About Target gene
| Target gene : |
Pax3(CDHS,HUP2,WS1,WS3) |
| Strong evidence: |
qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: |
RNA-Seq |
| Target gene experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About TF
| TF name : |
-- |
| TF experiment: |
Immunohistochemical analysis?Real-time PCR |
| TF experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About Function
| Enhancer function : |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
| Enhancer function experiment: |
Immunohistochemical staining |
Enhancer function
experiment description: |
We discovered that myoblast determining factor (MyoD) binding elements play a key role and participate in the transcriptional regulation of MYHM2528 1 expression in zebrafish embryos. We further discovered that paired box protein (Pax3) are required for promoting MYHM2528 1 expression and myocyte enhancer factor- 2 (MEF2) binding sites participate in the transcriptional regulation of MYHM2528 1 expression in slow/fast skeletal muscles. Our study also confirmed that the nuclear factor of activated T-cell (NFAT) binding sites take part in the transcriptional regulation of MYHM2528 1 expression in slow and fast muscles fiber in relation to indeterminate muscle growth. |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs