About Enhancer
| Enhancer ID: | E_01_0763 |
| Species: | human |
| Position : | chr17:48721960-48723960   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Glioma, breast cancer |
| Pubmed ID: | 30105866 |
| Enhancer experiment: | QRT PCR, chromatin immunoprecipitation (chip) assay, Weston blot, statistical analysis, gene knockdown |
| Enhancer experiment description: | Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. ;Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. |
About TF
| TF name : | HOXB13EZH2(ENX-1,ENX1b,KMT6,KMT6A,WVS,WVS2,EZH2) |
| TF experiment: | qRTPCR,???????(ChIP)??,weston blot,????,???? |
| TF experiment description: | Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. ;Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. |
About Function
| Enhancer function : | Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Conversely, knockdown of HOXB13 AS1 resulted in decreased cell proliferation and tumor growth. Mechanistically, we showed that HOXB13 AS1 overexpression increased DNMT3B mediated methylation of adjacent gene HOXB13 promoter by binding with the enhancer of zeste homolog 2 (EZH2) using bisulfite sequencing PCR (BSP), epigenetically suppressing HOXB13 expression. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| HOXB13 | Regulation of Androgen receptor activity | pid | 54 |
| EZH2 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| EZH2 | Oxidative Stress Induced Senescence | reactome | 120 |
| EZH2 | PKMTs methylate histone lysines | reactome | 64 |
| EZH2 | PRC2 methylates histones and DNA | reactome | 71 |
| EZH2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| EZH2 | Hs_Interactome_of_polycomb_repressive_complex_2_(PRC2)_WP2916_88672 | wikipathways | 15 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene