About Enhancer
| Enhancer ID: |
E_01_0517 |
| Species: |
human |
| Position :
|
chr19:6658582-6660582
  |
| Biosample name: |
|
| Experiment class :
|
High+Lowthroughput |
| Enhancer type: |
Enhancer |
| Disease: |
Skin pigmentation disturbances |
| Pubmed ID: |
29730385
|
| Enhancer experiment: |
RNA-seq,transfection,RT-qPCR,Cell proliferation assay,Western blot, |
| Enhancer experiment description: |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About Target gene
| Target gene : |
TNFSF14 |
| Strong evidence: |
qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: |
RNA-Seq |
| Target gene experiment description: |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About TF
| TF name : |
-- |
| TF experiment: |
RNA-seq,transfection,RT-qPCR,Cell proliferation assay,Western blot, |
| TF experiment description: |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About Function
| Enhancer function : |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
| Enhancer function experiment: |
Immunohistochemical staining |
Enhancer function
experiment description: |
To explore signaling in melanocytes treated with TNFSF14, we performed RNA-seq and found that nuclear factor ?-light-chain-enhancer of activated B cells (NF-?B) signaling is activated by TNFSF14. Further, we observed that inhibition of NF-kB effectively blocks the hypopigmentation induced by TNFSF14. We conclude that TNFSF14 inhibits melanogenesis in melanocytes via NF-?B signaling and could be applied in the treatment of cutaneous pigment disorders. |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs