About Enhancer

Enhancer ID: E_01_0308
Species: human
Position : chr9:117701433-117703433
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Periodontal disease
Pubmed ID:  30664173
Enhancer experiment: PCR, Western blot, sequencing technology
Enhancer experiment description: The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll?like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK?8 assays and ELISAs, respectively.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll?like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK?8 assays and ELISAs, respectively.

About TF

TF name : TLR4
TF experiment: PCR?Western blot?????
TF experiment description: The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll?like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK?8 assays and ELISAs, respectively.

About Function

Enhancer function : The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll?like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK?8 assays and ELISAs, respectively.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll?like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK?8 assays and ELISAs, respectively.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
TLR4 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon reactome 18
TLR4 Endogenous TLR signaling pid 26
TLR4 IKK complex recruitment mediated by RIP1 reactome 24
TLR4 IL-1_signaling(through_IKK-NFkB_cascade)(canonical) ( IL-1 signaling pathway (through NF-kappaB) ) inoh 15
TLR4 IRAK4 deficiency (TLR2/4) reactome 11
TLR4 Ligand-dependent caspase activation reactome 16
TLR4 MyD88-independent TLR3/TLR4 cascade reactome 7
TLR4 MyD88 deficiency (TLR2/4) reactome 11
TLR4 MyD88:Mal cascade initiated on plasma membrane reactome 19
TLR4 Toll-like receptor signaling pathway (p38 cascade) ( Toll-like receptor signaling pathway (p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ) inoh 15
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through JNK cascade)(Canonical) ( Toll-like receptor signaling pathway (through JNK cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through LPS, TLR4, MyD88, IRAK, TAK1 and IKK-NF-kappaB cascade)(Canonical) ( Toll-like receptor signaling pathway (trough NF-kappaB) ) inoh 19
TLR4 Toll Like Receptor 4 (TLR4) Cascade reactome 15
TLR4 Toll receptor signaling pathway panther 44
TLR4 TRAF6 mediated induction of TAK1 complex reactome 16
TLR4 TRIF-mediated programmed cell death reactome 10
TLR4 Phagosome kegg 154
TLR4 Toll-like receptor signaling pathway kegg 100
TLR4 Pathogenic Escherichia coli infection kegg 49
TLR4 Leishmaniasis kegg 71
TLR4 Chagas disease kegg 103
TLR4 Malaria kegg 50
TLR4 Toxoplasmosis kegg 131
TLR4 Amoebiasis kegg 103
TLR4 Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
TLR4 Hs_Regulation_of_toll-like_receptor_signaling_pathway_WP1449_81172 wikipathways 47
TLR4 Hs_Pathogenic_Escherichia_coli_infection_WP2272_78594 wikipathways 7
TLR4 Hs_Toll-like_Receptor_Signaling_WP3858_89945 wikipathways 7

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs