About Enhancer

Enhancer ID: E_01_0227
Species: human
Position : chr6:131945597-131947597
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Tumour
Pubmed ID:  31610176
Enhancer experiment: Flow cytometry,PCR,Western blot
Enhancer experiment description: Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.

About Target gene

Target gene : Sox2(ANOP3,MCOPS3),ZFHX4
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.;Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.;Although drugs, including those targeting specific mutations (eg, in BRAF) and checkpoint inhibitors, have been discovered that can retard melanoma progression in some patients, ultimately patients develop resistance to these regimens.;This resulted in a 9-gene score composed of the following genes: ZFHX4, DCN, ITGA11, COL6A3, COL1A1, ITGBL1, COL8A1, INHBA, and, MEG3. CAF-specific CCN2 scores were dichotimized with 13 receiver operating characteristics (ROC) curves to determine the optimal cutoff for the endpoint of overall survival censorship.

About TF

TF name : CCN2BRAF
TF experiment: Flow cytometry,PCR,Western blot
TF experiment description: Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.;Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.;Although drugs, including those targeting specific mutations (eg, in BRAF) and checkpoint inhibitors, have been discovered that can retard melanoma progression in some patients, ultimately patients develop resistance to these regimens.;This resulted in a 9-gene score composed of the following genes: ZFHX4, DCN, ITGA11, COL6A3, COL1A1, ITGBL1, COL8A1, INHBA, and, MEG3. CAF-specific CCN2 scores were dichotimized with 13 receiver operating characteristics (ROC) curves to determine the optimal cutoff for the endpoint of overall survival censorship.

About Function

Enhancer function : Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Conversely, fibroblast activation was impaired in mice with a fibroblast-specific deletion of Ccn2, associated with reduced expression of alpha-smooth muscle actin and Sox2.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
BRAF AKT(PKB)-Bad signaling ( EPO signaling pathway(JAK2 STAT1 STAT3 STAT5) ) inoh 173
BRAF AKT(PKB)-Bad signaling ( IL-7 signaling(JAK1 JAK3 STAT5) ) inoh 173
BRAF Angiogenesis panther 141
BRAF ARMS-mediated activation reactome 7
BRAF BCR netpath 161
BRAF CDC42 signaling events pid 71
BRAF CREB phosphorylation through the activation of Ras reactome 13
BRAF Downstream signaling in nave CD8+ T cells pid 69
BRAF Drosophila Toll-like receptor signaling ( Drosophila Toll-like receptor signaling ) inoh 236
BRAF EGFR1 netpath 475
BRAF ErbB1 downstream signaling pid 105
BRAF ERK cascade ( B cell receptor signaling ) inoh 7
BRAF ERK cascade ( CD4 T cell receptor signaling (ERK cascade) ) inoh 10
BRAF ERK cascade ( CD4 T cell receptor signaling ) inoh 7
BRAF ERK cascade ( FGF signaling pathway ) inoh 8
BRAF ERK cascade ( FGF8 signaling (Mouse) ) inoh 17
BRAF ERK cascade ( FGF8 signaling (Xenopus) ) inoh 17
BRAF ERK cascade ( GPCR Adenosine A2A receptor signaling pathway ) inoh 5
BRAF ERK cascade ( GPCR GroupI metabotropic glutamate receptor signaling pathway ) inoh 7
BRAF ERK cascade ( GPCR signaling (G alpha q) ) inoh 7
BRAF ERK cascade ( GPCR signaling (G alpha s, Epac and ERK) ) inoh 5
BRAF ERK cascade ( IGF1 signaling pathway ) inoh 8
BRAF ERK cascade ( Insulin receptor signaling (D. melanogaster) ) inoh 8
BRAF ERK cascade ( Insulin receptor signaling (Mammal) ) inoh 8
BRAF ERK cascade ( JAK-STAT pathway and regulation pathway Diagram ) inoh 7
BRAF ERK cascade ( NGF signaling pathway ) inoh 8
BRAF FGF signaling pathway ( FGF signaling pathway ) inoh 44
BRAF FGF8 signaling pathway(Mouse) ( FGF8 signaling (Mouse) ) inoh 20
BRAF FGF8 signaling pathway(Xenopus) ( FGF8 signaling (Xenopus) ) inoh 18
BRAF Frs2-mediated activation reactome 9
BRAF Heterotrimeric GPCR signaling pathway (through G alpha q, PLC beta and ERK cascade) ( GPCR signaling (G alpha q) ) inoh 239
BRAF Heterotrimeric GPCR signaling pathway (through glutamate, G alpha q and PLC beta) ( GPCR GroupI metabotropic glutamate receptor signaling pathway ) inoh 18
BRAF IGF1 signaling pathway ( IGF1 signaling pathway ) inoh 27
BRAF Insulin receptor signaling ( Insulin receptor signaling (D. melanogaster) ) inoh 55
BRAF Insulin receptor signaling (Mammal) ( Insulin receptor signaling (Mammal) ) inoh 58
BRAF Integrin signaling pathway ( Integrin signaling pathway ) inoh 123
BRAF Integrin signalling pathway panther 156
BRAF Interleukin signaling pathway panther 86
BRAF Intracellular Signalling Through Adenosine Receptor A2a and Adenosine smpdb 37
BRAF Intracellular Signalling Through Adenosine Receptor A2b and Adenosine smpdb 38
BRAF MAP2K and MAPK activation reactome 38
BRAF mTOR signaling pathway pid 66
BRAF Negative feedback regulation of MAPK pathway reactome 6
BRAF Negative regulation of MAPK pathway reactome 38
BRAF NGF signaling pathway ( NGF signaling pathway ) inoh 16
BRAF PDGF signaling pathway panther 113
BRAF PDGFR-beta signaling pathway pid 125
BRAF Positive regulation of (Transcription of SOCS by STAT dimer) in JAK STAT pathway ( JAK-STAT pathway and regulation pathway Diagram ) inoh 178
BRAF Prolactin netpath 105
BRAF RAF activation reactome 25
BRAF Raf activation signaling (through Grb2 and Sos) ( B cell receptor signaling ) inoh 9
BRAF Raf activation signaling (through Grb2 and Sos) ( CD4 T cell receptor signaling (ERK cascade) ) inoh 9
BRAF Raf activation signaling (through Grb2 and Sos) ( CD4 T cell receptor signaling ) inoh 9
BRAF Raf activation signaling (through Grb2 and Sos) ( JAK-STAT pathway and regulation pathway Diagram ) inoh 9
BRAF Raf activation signaling (through RasGRP) ( CD4 T cell receptor signaling (ERK cascade) ) inoh 8
BRAF Raf activation signaling (through RasGRP) ( CD4 T cell receptor signaling ) inoh 7
BRAF Ras Pathway panther 69
BRAF Ras signaling in the CD4+ TCR pathway pid 14
BRAF Signaling events mediated by focal adhesion kinase pid 63
BRAF Signaling events mediated by VEGFR1 and VEGFR2 pid 69
BRAF Signalling to p38 via RIT and RIN reactome 5
BRAF Spry regulation of FGF signaling reactome 16
BRAF T cell activation panther 60
BRAF Trk receptor signaling mediated by the MAPK pathway pid 34
BRAF VEGF signaling pathway ( VEGF signaling pathway ) inoh 183
BRAF VEGF signaling pathway panther 54
BRAF ERK cascade ( Integrin signaling pathway ) inoh 5
BRAF MAPK signaling pathway kegg 264
BRAF ErbB signaling pathway kegg 83
BRAF Chemokine signaling pathway kegg 187
BRAF mTOR signaling pathway kegg 50
BRAF Vascular smooth muscle contraction kegg 114
BRAF Focal adhesion kegg 197
BRAF Natural killer cell mediated cytotoxicity kegg 125
BRAF Long-term potentiation kegg 67
BRAF Neurotrophin signaling pathway kegg 126
BRAF Long-term depression kegg 67
BRAF Regulation of actin cytoskeleton kegg 210
BRAF Insulin signaling pathway kegg 134
BRAF Hepatitis C kegg 134
BRAF Pathways in cancer kegg 321
BRAF Colorectal cancer kegg 63
BRAF Renal cell carcinoma kegg 65
BRAF Pancreatic cancer kegg 70
BRAF Endometrial cancer kegg 52
BRAF Glioma kegg 65
BRAF Thyroid cancer kegg 25
BRAF Melanoma kegg 69
BRAF Bladder cancer kegg 38
BRAF Chronic myeloid leukemia kegg 69
BRAF Non-small cell lung cancer kegg 54
BRAF Hs_Cell_Cycle_WP179_89516 wikipathways 33
BRAF Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
BRAF Hs_MAPK_Signaling_Pathway_WP382_79951 wikipathways 58
BRAF Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
BRAF Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
BRAF Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
BRAF Hs_Bladder_Cancer_WP2828_89143 wikipathways 17
BRAF Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
BRAF Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
BRAF Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 wikipathways 71

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs